Background: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.
Description: Rabbit polyclonal to HMG-CoA reductase (pSer871)
Immunogen: A synthesized phosphopeptide derived from HMG-CoA reductase around the phosphorylation site of serine (N-R-SP-K-I).
Specificity: ·Reacts with Human, Mouse, Hamster and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/500-1000. Predicted Mol wt: 97 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/100-500;
·Immunocytochemistry: 1/200-500;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
